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RUNX2 Recombinant antibody

RUNX2 Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1G5

Cat no : 82636-2-RR

Synonyms

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RUNX2 Recombinant antibody (82636-2-RR)

    WB analysis using 82636-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82636-2-RR (RUNX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of ROS1728 cells using RUNX2 Recombinant antibody (82636-2-RR)

    IP experiment of ROS1728 using 82636-2-RR

    IP result of anti-RUNX2 (IP:82636-2-RR, 4ug; Detection:82636-2-RR 1:500) with ROS1728 cells lysate 2080 ug.

Tested Applications

Positive WB detected inPC-3 cells, Saos-2 cells, C2C12 cells
Positive IP detected inROS1728 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

82636-2-RR targets RUNX2 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name runt-related transcription factor 2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight57-60 kDa
GenBank Accession NumberNM_004348
Gene Symbol RUNX2
Gene ID (NCBI) 860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.

Protocols

Product Specific Protocols
WB protocol for RUNX2 antibody 82636-2-RRDownload protocol
IP protocol for RUNX2 antibody 82636-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci

Circ_0114581 promotes osteogenic differentiation of BMSCs via the MiR-155-5p/HNRNPA3 axis

Authors - Hao Li
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Udesh (Verified Customer) (11-26-2023)

Worked well for ADSCs at 1:1000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ADSCs