Tested Applications
| Positive WB detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
27099-1-AP targets RUNX3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24339 Product name: Recombinant human RUNX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-251 aa of BC013362 Sequence: PFPDRFGDLERLRMRVTPSTPSPRGSLSTTSHFSSQPQTPIQGTS Predict reactive species |
| Full Name | runt-related transcription factor 3 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 45-55 kDa |
| GenBank Accession Number | BC013362 |
| Gene Symbol | RUNX3 |
| Gene ID (NCBI) | 864 |
| RRID | AB_2880754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13761 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RUNX3, also known as AML2, CBFA3 and PEBP2A3, is a member of the RUNX family of transcription factors, which are crucial regulators of embryonic development and play key roles in various biological processes such as cell proliferation, apoptosis, differentiation and lineage commitment (PMID: 38430423). This protein functions as a tumour suppressor and is frequently deleted or transcriptionally silenced in various cancers, including gastric and colon cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RUNX3 antibody 27099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids Circular RNA circREPS2 Acts as a Sponge of miR-558 to Suppress Gastric Cancer Progression by Regulating RUNX3/β-catenin Signaling. | ||
Biochim Biophys Acta Mol Cell Res Lemur tyrosine kinase 2 has a tumor-inhibition function in human glioblastoma by regulating the RUNX3/Notch pathway | ||
Biochim Biophys Acta Mol Basis Dis A novel Cdc42-YAP-fibronectin signaling axis regulates ameloblast differentiation during early enamel formation |
