RUNX3 Polyclonal antibody

RUNX3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27099-1-AP

Synonyms

AML2, CBF alpha 3, CBFA3, Oncogene AML 2, PEA2 alpha C, PEBP2 alpha C, PEBP2A3, PEBP2aC, RUNX3

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Validation Data Gallery

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  WB analysis using 27099-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27099-1-AP (RUNX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse spleen tissue, human tonsillitis tissue, mouse small intestine tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

27099-1-AP targets RUNX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RUNX3 fusion protein Ag24339
• Full Name: runt-related transcription factor 3
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC013362
• Gene Symbol: RUNX3
• Gene ID (NCBI): 864
• RRID: AB_2880754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Circular RNA circREPS2 Acts as a Sponge of miR-558 to Suppress Gastric Cancer Progression by Regulating RUNX3/β-catenin Signaling.; Authors - Xiong Guo
human	WB	Biochim Biophys Acta Mol Cell Res; Lemur tyrosine kinase 2 has a tumor-inhibition function in human glioblastoma by regulating the RUNX3/Notch pathway; Authors - Lei Zhang