RUNX3 Polyclonal antibody

RUNX3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27099-1-AP

Synonyms

Oncogene AML 2, Core-binding factor subunit alpha-3, CBF-alpha-3, CBFA3, CBF alpha 3

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Validation Data Gallery

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  WB analysis using 27099-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27099-1-AP (RUNX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 27099-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 27099-1-AP (RUNX3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse spleen tissue, human tonsillitis tissue, mouse small intestine tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

27099-1-AP targets RUNX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RUNX3 fusion protein Ag24339
• Full Name: runt-related transcription factor 3
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC013362
• Gene Symbol: RUNX3
• Gene ID (NCBI): 864
• RRID: AB_2880754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUNX3, also known as AML2, CBFA3 and PEBP2A3, is a member of the RUNX family of transcription factors, which are crucial regulators of embryonic development and play key roles in various biological processes such as cell proliferation, apoptosis, differentiation and lineage commitment (PMID: 38430423). This protein functions as a tumour suppressor and is frequently deleted or transcriptionally silenced in various cancers, including gastric and colon cancer.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Circular RNA circREPS2 Acts as a Sponge of miR-558 to Suppress Gastric Cancer Progression by Regulating RUNX3/β-catenin Signaling.; Authors - Xiong Guo
human	WB	Biochim Biophys Acta Mol Cell Res; Lemur tyrosine kinase 2 has a tumor-inhibition function in human glioblastoma by regulating the RUNX3/Notch pathway; Authors - Lei Zhang
mouse	IHC	Biochim Biophys Acta Mol Basis Dis; A novel Cdc42-YAP-fibronectin signaling axis regulates ameloblast differentiation during early enamel formation; Authors - Jiayi Zhang