RXRG Polyclonal antibody

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• RXRG Polyclonal antibody (11129-1-AP)

RXRG Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11129-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA, PLA

Synonyms

RXRC, Retinoic acid receptor RXR-gamma, NR2B3

• 

  WB analysis of Jurkat using 11129-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 11129-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat eye using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat eye using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse ovary tissue
• Positive IHC detected in: rat eye tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11129-1-AP targets RXRG in WB, IHC, IF, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1611

  Product name: Recombinant human RXRG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 164-463 aa of BC012063

  Sequence: KRTIRKDLIYTCRDNKDCLIDKRQRNRCQYCRYQKCLVMGMKREAVQEERQRSRERAESEAECATSGHEDMPVERILEAELAVEPKTESYGDMNMENSTNDPVTNICHAADKQLFTLVEWAKRIPHFSDLTLEDQVILLRAGWNELLIASFSHRSVSVQDGILLATGLHVHRSSAHSAGVGSIFDRVLTELVSKMKDMQMDKSELGCLRAIVLFNPDAKGLSNPSEVETLREKVYATLEAYTKQKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLETPLQIT

• Full Name: retinoid X receptor, gamma
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC012063
• Gene Symbol: RXRG
• Gene ID (NCBI): 6258
• RRID: AB_2182635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48443
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RXRG, also named as, Retinoic acid receptor RXR-gamma, is a 463 amino acid protein, which localize in the nucleus and may form homodimer. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5.

Publications

What published studies show

The RXRG Polyclonal antibody (Cat# 11129-1-AP) has 6 citations published in PNAS, Journal of Medicinal Chemistry, Cells, Aging, Journal of Cellular and Molecular Medicine, and American Journal of Physiology – Lung Cellular and Molecular Physiology. Research fields span oncology (small-cell lung cancer, prostate cancer), neuroscience (oligodendrocyte maturation), muscle biology (vitamin D3 and myotube atrophy), cardiovascular disease (cerebral small vessel disease), and pulmonology (neonatal lung injury).

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Phase separation of RXRγ drives tumor chemoresistance and represents a therapeutic target for small-cell lung cancer; Authors - Hong Wang ; KD Validated
human	WB	J Med Chem; Discovery of a Potent RXRγ Degrader WCF-598 for the Treatment of Castration-Resistant Prostate Cancer.; Authors - Chaofan Wang ; KD Validated
human	WB	Cells; EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP.; Authors - Emanuela Nocita
mouse	PLA	Aging (Albany NY); Cholecalciferol (vitamin D3) has a direct protective activity against interleukin 6-induced atrophy in C2C12 myotubes.; Authors - Maraiza Alves Teixeira
rat	WB	J Cell Mol Med; Daqinjiao Decoction Ameliorates CSVD via RXR-γ/PPAR-γ/VEGF-α Pathway: Insights From Transcriptome Sequencing and Network Pharmacology; Authors - Mengna Lv
rat	IF	Am J Physiol Lung Cell Mol Physiol; Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.; Authors - Craig A Gelfand