RXRG Monoclonal antibody

RXRG Monoclonal Antibody for WB, ELISA

Cat No. 66306-1-Ig

Clone No. 1E7E5

Publications (1)

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Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

NR2B3, Retinoid X receptor gamma, retinoid X receptor, gamma, RXRC, RXRG

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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WB analysis of A549 using 66306-1-Ig
A549 cells were subjected to SDS PAGE followed by western blot with 66306-1-Ig (RXRG Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66306-1-Ig targets RXRG in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag1611 Product name: Recombinant human RXRG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 164-463 aa of BC012063 Sequence: KRTIRKDLIYTCRDNKDCLIDKRQRNRCQYCRYQKCLVMGMKREAVQEERQRSRERAESEAECATSGHEDMPVERILEAELAVEPKTESYGDMNMENSTNDPVTNICHAADKQLFTLVEWAKRIPHFSDLTLEDQVILLRAGWNELLIASFSHRSVSVQDGILLATGLHVHRSSAHSAGVGSIFDRVLTELVSKMKDMQMDKSELGCLRAIVLFNPDAKGLSNPSEVETLREKVYATLEAYTKQKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLETPLQIT Predict reactive species
Full Name	retinoid X receptor, gamma
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	51 kDa
GenBank Accession Number	BC012063
Gene Symbol	RXRG
Gene ID (NCBI)	6258
RRID	AB_2881688
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P48443
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RXRG, also named as, Retinoic acid receptor RXR-gamma, is a 463 amino acid protein, which localize in the nucleus and may form homodimer. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5.

Protocols

Product Specific Protocols
WB protocol for RXRG antibody 66306-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The antibody (catalog #66306-1-Ig) has 1 total citation. It was cited in Cell Death & Disease (IF 12.2), in a study investigating how p53 induces ARTS to promote mitochondrial apoptosis in human samples. The associated research field is apoptosis and cell death signaling.