RYBP Polyclonal antibody

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  WB analysis of K-562 using 11365-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11365-1-AP (RYBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 11365-1-AP

  IP result of anti-RYBP (IP:11365-1-AP, 4ug; Detection:11365-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

RYBP Polyclonal Antibody for WB, IP, ELISA

Cat No. 11365-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11365-1-AP targets RYBP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1908

  Product name: Recombinant human RYBP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-228 aa of BC014959

  Sequence: MTMGDKKSPTRPKRQAKPAADEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESF

• Full Name: RING1 and YY1 binding protein
• Calculated Molecular Weight: 228 aa, 25 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014959
• Gene Symbol: RYBP
• Gene ID (NCBI): 23429
• RRID: AB_2285461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N488
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan