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- KD/KO Validated
RYK Polyclonal antibody
RYK Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 22138-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, A549 cells, mouse brain tissue, SH-SY5Y cells |
Positive IHC detected in | human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
Product Information
The immunogen of 22138-1-AP is RYK Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RYK fusion protein Ag17540 |
Full Name | RYK receptor-like tyrosine kinase |
Calculated Molecular Weight | 610 aa, 68 kDa |
Observed Molecular Weight | 67-75 kDa |
GenBank Accession Number | BC153090 |
Gene Symbol | RYK |
Gene ID (NCBI) | 6259 |
RRID | AB_11232602 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for RYK antibody 22138-1-AP | Download protocol |
IHC protocol for RYK antibody 22138-1-AP | Download protocol |
IF protocol for RYK antibody 22138-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Neuroinflammation Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model | ||
Cell Mol Biol Lett Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway | ||
Pharmaceuticals (Basel) RYK Gene Expression Associated with Drug Response Variation of Temozolomide and Clinical Outcomes in Glioma Patients |