Rad51D Polyclonal antibody

Rad51D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30068-1-AP

Synonyms

HsTRAD, R51H3, RAD51 homolog D, RAD51 like 3 (S. cerevisiae), RAD51 like protein 3, Rad51D, RAD51L3, Trad

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Validation Data Gallery

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  WB analysis using 30068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30068-1-AP (Rad51D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 30068-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 30068-1-AP (Rad51D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells, A2780 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30068-1-AP targets Rad51D in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Rad51D fusion protein Ag32303
• Full Name: RAD51-like 3 (S. cerevisiae)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC014422
• Gene Symbol: RAD51L3
• Gene ID (NCBI): 5892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD51L3, also named RAD51D, R51H3 and TRAD, belongs to the recA family and RAD51 subfamily. It is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA, and specifically to nicks in duplex DNA. The Heterodimer of RAD51L3 is about 70 kDa.