Speedy™ Rat AGER/RAGE One-Step ELISA Kit
Cat no : SE70003
Synonyms
AGER, RAGE, Advanced glycosylation end product-specific receptor
Validation Data Gallery
Product Information
| Product name | Speedy™ Rat AGER/RAGE One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 3.6 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81722 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 91% | 81%-97% |
| Urine | 90% | 86%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,087.7 | 26.9 | 2.5 |
| 2 | 8 | 258.6 | 3.4 | 1.3 |
| 3 | 8 | 146.2 | 2.2 | 1.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,134.4 | 56.6 | 5.0 |
| 2 | 16 | 261.1 | 4.9 | 1.9 |
| 3 | 16 | 149.4 | 4.2 | 2.8 |
Background Information
RAGE is a multiligand receptor belonging to the immunoglobulin superfamily of cell surface receptors. It is highly expressed in inflammation-related pathological states and is associated with severe chronic pathologies, including diabetic complications, chronic inflammation, and cancer. Soluble forms of RAGE (sRAGE) are produced by proteolytic cleavage of full-length RAGE and alternative mRNA splicing. sRAGE can antagonize full-length RAGE and other receptors by binding DAMPs and other ligands, inhibiting leukocyte recruitment in various acute and chronic inflammatory conditions. sRAGE also has been identified as possessing therapeutic potential, as it can alleviate myocardial fibrosis and suppress autophagy and ischemia/reperfusion injury in rodent studies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGER, RAGE, Advanced glycosylation end product-specific receptor |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.