Rat C-Reactive Protein/CRP ELISA Kit
Cat no : KE20029
Synonyms
C-Reactive Protein,CRP,PTX 1,PTX1
Validation Data Gallery
Product Information
KE20029 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat C-Reactive Protein/CRP ELISA kit is to be used to detect and quantify protein levels of endogenous rat C-Reactive Protein/CRP. The assay recognizes rat C-Reactive Protein/CRP. An antibody specific for rat C-Reactive Protein/CRP has been pre-coated onto the microwells. The rat C-Reactive Protein/CRP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat C-Reactive Protein/CRP is added to detect the captured rat C-Reactive Protein/CRP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat C-Reactive Protein/CRP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 25419 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 114% | 101%-129% |
| Cell culture supernatant | 109% | 101%129% |
| Tissue lysate | 99% | 92%-108% |
| Urine | 105% | 100%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,358.9 | 82.0 | 6.0 |
| 2 | 20 | 290.1 | 6.3 | 2.2 |
| 3 | 20 | 71.2 | 2.8 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,156.1 | 107.2 | 9.3 |
| 2 | 24 | 271.3 | 15.0 | 5.5 |
| 3 | 24 | 71.7 | 3.1 | 4.3 |
Background Information
C-reactive protein (CRP) is an evolutionary highly conserved member of the pentraxin superfamily of proteins. CRP consists of five identical subunits with molecular weight 20-30 kDa arranged as a cyclic pentamer. CRP exists in at least two conformationally distinct forms, native pentameric CRP (pCRP) and modified/monomeric CRP (mCRP). CRP plays important roles in inflammatory processes and host responses to infection including promoting agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its Ca2+-dependent binding to phosphorylcholine (PCh). CRP is mainly expressed by hepatocytes and secreted into plasma. The concentration of CRP will increase significantly in plasma during acute phase response to tissue injury, infection, or other inflammatory stimuli.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C-Reactive Protein,CRP,PTX 1,PTX1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.