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Rat CCL20/MIP-3 alpha Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: rat

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat no : MP02533-2

Synonyms

CCL20, MIP-3 Alpha, MIP-3α, Beta-chemokine exodus-1, C C motif chemokine 20

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02533-2, Rat CCL20/MIP-3 alpha Recombinant Matched Antibody Pair - PBS only. 86607-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3237. 86607-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP02533-2

    Sandwich ELISA standard curve of MP02533-2, Rat CCL20/MIP-3 alpha Recombinant Matched Antibody Pair - PBS only. 86607-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3237. 86607-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • The mean CCL20/MIP-3 alpha concentration was determined to be 7.3 ng/mL in rat brain tissue extract based on a 1.6 mg/mL extract load.

    Sandwich ELISA sample test of MP02533-2

    The mean CCL20/MIP-3 alpha concentration was determined to be 7.3 ng/mL in rat brain tissue extract based on a 1.6 mg/mL extract load.

  • Rat spleen, cut into 1-2 mm pieces, was cultured unstimulated or stimulated with 5 μg/mL ConA for 2 days. The mean CCL20/MIP-3 alpha concentration was undetectable in unstimulated supernatant, 94.6 pg/mL in stimulated supernatant.

    Sandwich ELISA sample test of MP02533-2

    Rat spleen, cut into 1-2 mm pieces, was cultured unstimulated or stimulated with 5 μg/mL ConA for 2 days. The mean CCL20/MIP-3 alpha concentration was undetectable in unstimulated supernatant, 94.6 pg/mL in stimulated supernatant.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02533-2 targets CCL20/MIP-3 alpha in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: Rat CCL20/MIP-3 alpha Recombinant monoclonal antibody, PBS Only (Capture) 86607-1-PBS (250116F2). 100 μg. Concentration 1 mg/ml.

Detection antibody: Rat CCL20/MIP-3 alpha Recombinant monoclonal antibody, PBS Only (Detector) 86607-2-PBS (250116D3). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg3237

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86607-1-PBS
Tested Reactivity rat
Immunogen CCL20/MIP-3 alpha fusion protein Eg3237
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 20
Synonyms Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20
GenBank accession numberNM_019233.1
Gene symbol Ccl20
UniprotP97884
Gene ID (NCBI) 29538
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86607-2-PBS
Tested Reactivity rat
Immunogen CCL20/MIP-3 alpha fusion protein Eg3237
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 20
Synonyms Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20
GenBank accession numberNM_019233.1
Gene symbol Ccl20
UniprotP97884
Gene ID (NCBI) 29538
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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