Rat CXCL1 ELISA Kit
Cat no : KE20015
Synonyms
Cinc1, CINC-1, C-X-C motif chemokine 1, Cytokine-induced neutrophil chemoattractant 1, Gro
Validation Data Gallery
Product Information
KE20015 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL1 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL1. The assay recognizes rat CXCL1. An antibody specific for CXCL1 has been pre-coated onto the microwells. The CXCL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CXCL1 is added to detect the captured CXCL1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat CXCL1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.06 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81503 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 103% | 86%-118% |
| Cell culture supernatants | 95% | 77%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 118.3 | 5.4 | 4.6 |
| 2 | 20 | 29.3 | 0.9 | 3.2 |
| 3 | 20 | 6.6 | 0.2 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 114.2 | 6.1 | 5.3 |
| 2 | 24 | 29.7 | 1.3 | 4.2 |
| 3 | 24 | 7.6 | 0.7 | 9.0 |
Background Information
CXC motif chemokine ligand 1 (CXCL1), a CXC chemokine, is also known as growth-regulated (or-related) oncogene-α (Gro-α) . Its most important property is to cause chemotaxis of immune cells, mainly neutrophils, and to a lesser extent CD14+ monocytes and basophils , but not T cells. The main receptor for CXCL1 is CXCR2, activated at concentrations of just a few nanomoles of CXCL1. Any inflammatory response is associated with an increase in the level of pro-inflammatory cytokines which induce an increase in CXCL1 expression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cinc1, CINC-1, C-X-C motif chemokine 1, Cytokine-induced neutrophil chemoattractant 1, Gro |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
J Biol Chem Endothelin-1 induces Zfp36 family RNA binding proteins and restrains cytokine and chemokine production in reactive astrocytes. |