Rat CXCL1 ELISA Kit
Cat no : KE20033
Synonyms
Cinc1,CINC-1,C-X-C motif chemokine 1,Cxcl1,Cytokin
Validation Data Gallery
Product Information
KE20033 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat CXCL1 ELISA kit is to be used to detect and quantify protein levels of endogenous rat CXCL1. The assay recognizes rat CXCL1. An antibody specific for rat CXCL1 has been pre-coated onto the microwells. The rat CXCL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat CXCL1 is added to detect the captured rat CXCL1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat CXCL1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81503 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 77% | 70%-83% |
| Cell culture supernatant | 91% | 89%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 965.6 | 82.1 | 8.5 |
| 2 | 8 | 230.5 | 13.6 | 5.9 |
| 3 | 8 | 113.3 | 10.3 | 9.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 991.4 | 85.3 | 8.6 |
| 2 | 16 | 235.4 | 16.0 | 6.8 |
| 3 | 16 | 113.3 | 10.0 | 8.8 |
Background Information
CXC motif chemokine ligand 1 (CXCL1), a CXC chemokine, is also known as growth-regulated (or-related) oncogene-α (Gro-α) . Its most important property is to cause chemotaxis of immune cells, mainly neutrophils, and to a lesser extent CD14+ monocytes and basophils , but not T cells. The main receptor for CXCL1 is CXCR2, activated at concentrations of just a few nanomoles of CXCL1. Any inflammatory response is associated with an increase in the level of pro-inflammatory cytokines which induce an increase in CXCL1 expression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cinc1,CINC-1,C-X-C motif chemokine 1,Cxcl1,Cytokin |