Rat CXCL2 ELISA Kit
Cat no : KE20017
Synonyms
Cxcl2, Mip 2, Scyb2
Validation Data Gallery
Product Information
KE20017 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat CXCL2 ELISA kit is to be used to detect and quantify protein levels of endogenous rat CXCL2. The assay recognizes rat CXCL2. An antibody specific for rat CXCL2 has been pre-coated onto the microwells. The rat CXCL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat CXCL2 is added to detect the captured rat CXCL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat CXCL2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 114105 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 105% | 89%-129% |
| Cell culture supernatants | 110% | 94%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 227.4 | 11.9 | 5.3 |
| 2 | 20 | 56.1 | 1.6 | 2.8 |
| 3 | 20 | 13.6 | 0.5 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 221.1 | 10.8 | 4.9 |
| 2 | 24 | 55.9 | 2.2 | 3.9 |
| 3 | 24 | 13.2 | 0.8 | 5.7 |
Background Information
Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2. During inflammation, the level of CXCL2 increases and is involved in the release of opioid peptides from leukocytes. Down-regulation of CXCL2 could reduce the recruitment of polymorphonuclear cells, which are key players in the clearance of L. monocytogenes during infection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cxcl2, Mip 2, Scyb2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Biomed Pharmacother Therapeutic and prophylactic effects of baicalin on Pneumocystis infection in immunosuppressed rats |