Rat FABP1 ELISA Kit
Cat no : KE20011
Synonyms
Fabp1, Fabplg, RATFABPLG
Validation Data Gallery
Product Information
KE20011 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FABP1 ELISA kit is to be used to detect and quantify protein levels of endogenous FABP1. The assay recognizes rat FABP1. An antibody specific for FABP1 has been pre-coated onto the microwells. The FABP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat FABP1 is added to detect the captured rat FABP1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat FABP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 8.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24360 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 105% | 76%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,511.5 | 55.7 | 2.2 |
| 2 | 20 | 633.0 | 18.4 | 2.9 |
| 3 | 20 | 324.5 | 17.5 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,362.5 | 66.1 | 2.8 |
| 2 | 24 | 599.4 | 16.9 | 2.8 |
| 3 | 24 | 309.9 | 11.8 | 3.8 |
Background Information
Fatty acid‐binding protein 1 (FABP1, also known as L‐FABP) is a 14 KDa cytosolic protein abundantly expressed in the liver. It can bind fatty acids and many other molecules and is involved in the uptake, transport, and metabolism of cellular long-chain fatty acids and other lipid ligands. Higher FABP1 serum level has been associated with liver injury. Levels of FABP1 can be helpful as a biomarker for metabolic disturbances and impaired function of hepatocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Fabp1, Fabplg, RATFABPLG |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.