Rat FABP1 ELISA Kit

Sensitivity

8.1 pg/mL

Range

78.1-5000 pg/mL

Reactivity

Rat

Cat no : KE20011

Synonyms

Fabp1, Fabplg, RATFABPLG



Product Information

KE20011 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FABP1 ELISA kit is to be used to detect and quantify protein levels of endogenous FABP1. The assay recognizes rat FABP1. An antibody specific for FABP1 has been pre-coated onto the microwells. The FABP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat FABP1 is added to detect the captured rat FABP1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Rat FABP1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma
Assay type Sandwich
Sensitivity 8.1 pg/mL
Range 78.1-5000 pg/mL
Reactivity Rat
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Rat serum105%76%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202,511.555.72.2
220633.018.42.9
320324.517.55.4

InterAssay

Samplenmean ( pg/mL)SDCV%
1242,362.566.12.8
224599.416.92.8
324309.911.83.8

Background Information

Fatty acid‐binding protein 1 (FABP1, also known as L‐FABP) is a 14 KDa cytosolic protein abundantly expressed in the liver. It can bind fatty acids and many other molecules and is involved in the uptake, transport, and metabolism of cellular long-chain fatty acids and other lipid ligands. Higher FABP1 serum level has been associated with liver injury. Levels of FABP1 can be helpful as a biomarker for metabolic disturbances and impaired function of hepatocytes.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Fabp1, Fabplg, RATFABPLG