Rat Ferritin light chain 1 ELISA Kit
Cat no : KE20040
Synonyms
Ferritin L subunit 1, Ftl, Ftl1
Validation Data Gallery
Product Information
KE00385 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat Ferritin light chain 1 ELISA kit is to be used to detect and quantify protein levels of endogenous rat Ferritin light chain 1. The assay recognizes rat Ferritin light chain 1. An antibody specific for rat Ferritin light chain 1 has been pre-coated onto the microwells. The rat Ferritin light chain 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat Ferritin light chain 1 is added to detect the captured rat Ferritin light chain 1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat Ferritin light chain 1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.20 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 29292 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 88% | 82%-91% |
| Tissue lysate | 83% | 73%-89% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 15.44 | 0.86 | 5.58 |
| 2 | 8 | 3.74 | 0.24 | 6.44 |
| 3 | 8 | 2.09 | 0.10 | 4.66 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 15.56 | 1.14 | 7.31 |
| 2 | 16 | 3.73 | 0.18 | 4.83 |
| 3 | 16 | 2.05 | 0.15 | 7.30 |
Background Information
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ferritin L subunit 1, Ftl, Ftl1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.