Speedy™ Rat IL-2 One-Step ELISA Kit
Cat no : SE70001
Synonyms
Il2, interleukin 2
Validation Data Gallery
Product Information
| Product name | Speedy™ Rat IL-2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 2.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 116562 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 111% | 92%-108% |
| Cell culture supernatant | 90% | 74%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 272.5 | 9.0 | 3.3 |
| 2 | 8 | 69.1 | 5.0 | 7.2 |
| 3 | 8 | 34.7 | 1.7 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 271.8 | 8.1 | 3.0 |
| 2 | 16 | 68.6 | 4.3 | 6.2 |
| 3 | 16 | 34.1 | 2.2 | 6.6 |
Background Information
IL-2 (Interleukin-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It regulates white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells. IL-2 mediates its effects by binding to IL-2 receptors which are expressed by lymphocytes. IL-2 signal can be transduced by 3 different signaling pathways: JAK-STAT, PI3K/Akt/mTOR and MAPK/ERK pathway. IL-2 plays a role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. It also promotes proliferation of activated B cells and subsequently immunoglobulin production. IL-2 has been used to treat immune-deficiencies like HIV, and induce immune response against tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il2, interleukin 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.