Rat Insulin ELISA Kit

Sensitivity

0.2 ng/mL

Range

0.31-20 ng/mL

Reactivity

Rat

Cat no : KE20008

Synonyms

Ins1, insulin 1



Product Information

KE20008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Insulin ELISA kit is to be used to detect and quantify protein levels of endogenous Insulin. The assay recognizes Rat Insulin. An antibody specific for Insulin has been pre-coated onto the microwells. The Insulin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Insulin is added to detect the captured Insulin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

Product name Rat Insulin ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma
Assay type Sandwich
Sensitivity 0.2 ng/mL
Range 0.31-20 ng/mL
Reactivity Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 24505

Recovery

Sample TypeAverageRange
Rat serum107%90%-124%

IntraAssay

Samplenmean ( ng/mL)SDCV%
1200.50.046.6
2202.40.187.4
3209.20.687.4

InterAssay

Samplenmean ( ng/mL)SDCV%
1240.60.069.6
2242.40.156.0
3249.40.717.5

Background Information

Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Ins1, insulin 1

Publications

SpeciesSample TypeTitle
ratserum

Phytomedicine

Moringa oleifera Lam. seed extract protects kidney function in rats with diabetic nephropathy by increasing GSK-3β activity and activating the Nrf2/HO-1 pathway.

Authors - Ying Wen
ratserum

Front Microbiol

Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation.

Authors - Qing-Xiang Zheng
mouseplasma

J Agric Food Chem

Ameliorating Effect of the Total Flavonoids of Morus nigra L. on Prediabetic Mice Based on Regulation of Inflammation and Insulin Sensitization

Authors - Xinxiu Ren
ratsupernatant

Endokrynol Pol

Neu-P11, a novel melatonin receptor agonist, could improve the features of type-2 diabetes mellitus in rats.

Authors - Xiuping Li