Rat RAGE ELISA Kit
Cat no : KE20030
Synonyms
AGER,RAGE,Advanced glycosylation end product-speci
Validation Data Gallery
Product Information
KE20030 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat RAGE ELISA kit is to be used to detect and quantify protein levels of endogenous rat RAGE. The assay recognizes rat RAGE. An antibody specific for rat RAGE has been pre-coated onto the microwells. The rat RAGE protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat RAGE is added to detect the captured rat RAGE protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat RAGE ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 4.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81722 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 104% | 89%-121% |
| Rat urine | 106% | 103%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 540.7 | 20.9 | 3.9 |
| 2 | 20 | 126.1 | 4.7 | 3.8 |
| 3 | 20 | 31.7 | 1.5 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 486.9 | 32.6 | 6.7 |
| 2 | 24 | 120.2 | 6.6 | 5.5 |
| 3 | 24 | 31.0 | 1.3 | 4.3 |
Background Information
RAGE is a multiligand receptor belonging to the immunoglobulin superfamily of cell surface receptors. It is highly expressed in inflammation-related pathological states and is associated with severe chronic pathologies, including diabetic complications, chronic inflammation, and cancer. Soluble forms of RAGE (sRAGE) are produced by proteolytic cleavage of full-length RAGE and alternative mRNA splicing. sRAGE can antagonize full-length RAGE and other receptors by binding DAMPs and other ligands, inhibiting leukocyte recruitment in various acute and chronic inflammatory conditions. sRAGE also has been identified as possessing therapeutic potential, as it can alleviate myocardial fibrosis and suppress autophagy and ischemia/reperfusion injury in rodent studies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGER,RAGE,Advanced glycosylation end product-speci |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Animal Model Exp Med Metabolic and biochemical profiling reveals phenotypic heterogeneity in Zucker diabetic fatty rats. | ||
Hum Exp Toxicol Omarigliptin/shikonin combination alleviates cyclosporine-induced nephrotoxicity: The role of sirtuin 1, glucagon-like peptide-1, HMGB1/RAGE/TLR4 signaling, and p38/ERK/JNK MAPKs. |