Ribosomal protein L4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80959-1-RR (RPL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80959-1-RR (same clone as 80959-1-PBS)

  WB result of Ribosomal protein L4 antibody (80959-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HEK-293 cells. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

  IP result of anti-Ribosomal protein L4 (IP:80959-1-RR, 4ug; Detection:80959-1-RR 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 80959-1-RR (same clone as 80959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR (same clone as 80959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (80959-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Ribosomal protein L4 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 80959-1-PBS

Clone No.3K6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

RPL4, 3K6, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4

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Product Information

80959-1-PBS targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag28728

  Product name: Recombinant human RPL4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-427 aa of BC009888

  Sequence: MACARPLISVYSEKGESSGKNVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKLAPGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMINTDLSRILKSPEIQRALRAPRKKIHRRVLKKNPLKNLRIMLKLNPYAKTMRRNTILRQARNHKLRVDKAAAAAAALQAKSDEKAAVAGKKPVVGKKGKKAAVGVKKQKKPLVGKKAAATKKPAPEKKPAEKKPTTEEKKPAA

• Full Name: ribosomal protein L4
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC009888
• Gene Symbol: Ribosomal protein L4
• Gene ID (NCBI): 6124
• RRID: AB_2918922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P36578
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.