Rootletin Polyclonal antibody

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• Rootletin Polyclonal antibody (55485-1-AP)

Rootletin Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 55485-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, CoIP, ELISA

Synonyms

CROCC, KIAA0445, ROLT, Rootletin

• 

  WB analysis of mouse brain using 55485-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55485-1-AP (Rootletin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of hTERT-RPE1 using 55485-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Rootletin antibody (55485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55485-1-AP targets Rootletin in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ciliary rootlet coiled-coil, rootletin
• Calculated Molecular Weight: 229 kDa
• Observed Molecular Weight: 228 kDa
• GenBank Accession Number: NM_014675
• Gene Symbol: Rootletin
• Gene ID (NCBI): 9696
• RRID: AB_2935543
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5TZA2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rootletin is predicted to enable kinesin binding activity and structural molecule activity. Involved in several processes, including centriole-centriole cohesion; positive regulation of cilium assembly; and positive regulation of protein localization to cilium.

Publications

What published studies show

Antibody 55485-1-AP has 1 total citation. It appears in bioRxiv (2026), in a study on KDM4A's role in maintaining basal body architecture and protecting against ciliary destabilization. The research field is ciliary biology and cell structure. The antibody was applied in IF and CoIP using human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF,CoIP	bioRxiv; Lysine Demethylase 4A (KDM4A) Maintains Basal Body Architecture and Protects Against Ciliary Destabilization.; Authors - Manga Motrapu