RyDEN Polyclonal antibody, PBS Only

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• RyDEN Polyclonal antibody, PBS Only (27865-1-PBS)

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RyDEN Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27865-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

C19orf66, Interferon-regulated antiviral protein, IRAV, Repressor of yield of DENV protein, SFL

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  WB analysis of THP-1 using 27865-1-AP (same clone as 27865-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27865-1-AP (RyDEN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27865-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27865-1-AP (same clone as 27865-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27865-1-AP (RyDEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27865-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 27865-1-AP (same clone as 27865-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27865-1-AP (RyDEN antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27865-1-PBS in a different storage buffer formulation.

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Product Information

27865-1-PBS targets RyDEN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26838

  Product name: Recombinant human C19orf66 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-255 aa of BC035817

  Sequence: MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPEDMKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYEPVPADKMWGLAEFHCPKCRHNFRTHTHSCSAADCYNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEEEVEDEEGGPRE

• Full Name: chromosome 19 open reading frame 66
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC035817
• Gene Symbol: C19orf66
• Gene ID (NCBI): 55337
• RRID: AB_2880997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NUL5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The shiftless antiviral inhibitor of ribosomal frameshifting (SHFL) gene was firstly predicted as an ISG in 2011, which is also named as C19orf66, FLJ11286, IRAV, UPF0515, and RyDEN. SHFL showed high expressing level in human liver and spleen tissues, whereas a lower expression is observed in the pancreas and heart. SHFL is a novel ISG that inhibits dengue virus (DENV), West Nile virus (WNV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV) infections. Interestingly, SHFL functions as a broad-spectrum antiviral factor exhibiting suppressive activity against various types of RNA and DNA viruses. (PMID: 36293480, PMID: 26735137, PMID: 38149274)