S100A7/Psoriasin Polyclonal antibody

S100A7/Psoriasin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13061-1-AP

Synonyms

S100A7, S100 calcium-binding protein A7, Psoriasin, PSOR1, Protein S100-A7

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Validation Data Gallery

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  WB analysis of MCF-7 using 13061-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 13061-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using S100A7/Psoriasin antibody (13061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human oesophagus cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13061-1-AP targets S100A7/Psoriasin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A7/Psoriasin fusion protein Ag3738
• Full Name: S100 calcium binding protein A7
• Calculated Molecular Weight: 101 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC034687
• Gene Symbol: S100A7/Psoriasin
• Gene ID (NCBI): 6278
• RRID: AB_2877908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.

Publications

Species	Application	Title
human	WB,IHC	J Eur Acad Dermatol Venereol; TOPK promotes the development of psoriasis and worenine alleviates psoriasiform dermatitis by inhibiting TOPK activity; Authors - Hui Lu
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan
human	IHC	BMC Cancer; A novel metabolic subtype with S100A7 high expression represents poor prognosis and immuno-suppressive tumor microenvironment in bladder cancer; Authors - Yun Cai
human	WB	Cell Biol Int; S100A7 promotes the development of human endometriosis by activating NF-κB signaling pathway in endometrial stromal cells.; Authors - Qingqing Sun
human	WB	Oncol Lett; PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.; Authors - Yushen Su
human	WB	Mol Med Rep; TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.; Authors - Xinxin Liu