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  • KD/KO Validated

S100A8 Polyclonal antibody

S100A8 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15792-1-AP

Synonyms

60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A



Tested Applications

Positive WB detected inTHP-1 cells, A549 cells, COLO 320 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

15792-1-AP targets S100A8 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A8 fusion protein Ag8500
Full Name S100 calcium binding protein A8
Calculated molecular weight 93 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC005928
Gene symbol S100A8
Gene ID (NCBI) 6279
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).

Protocols

Product Specific Protocols
WB protocol for S100A8 antibody 15792-1-APDownload protocol
IHC protocol for S100A8 antibody 15792-1-APDownload protocol
FC protocol for S100A8 antibody 15792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Hexokinase 1 cellular localization regulates the metabolic fate of glucose.

Authors - Adam De Jesus
mouseWB,IHC

Nat Commun

S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.

Authors - Jianjie Li
humanWB

Mol Ther

Bulk and single-cell transcriptome profiling reveal the metabolic heterogeneity in human breast cancers.

Authors - Tian-Jian Yu
  • KD Validated
mouseWB

Pharmacol Res

Triptolide analog LLDT-8 ameliorates psoriasis-like dermatitis in BALB/c mice via suppressing the IL-36α signaling pathway.

Authors - Qing Qi
mouseWB, IHC

Oncotarget

Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation.

Authors - Zeqian Yu
humanWB,IF

Front Cell Dev Biol

BRG1 Stimulates Endothelial Derived Alarmin MRP8 to Promote Macrophage Infiltration in an Animal Model of Cardiac Hypertrophy.

Authors - Zilong Li