Validation Data Gallery
|Positive WB detected in||THP-1 cells, A549 cells, COLO 320 cells|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||RAW 264.7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
15792-1-AP targets S100A8 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100A8 fusion protein Ag8500|
|Full Name||S100 calcium binding protein A8|
|Calculated molecular weight||93 aa, 11 kDa|
|Observed molecular weight||11 kDa|
|GenBank accession number||BC005928|
|Gene ID (NCBI)||6279|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).
Hexokinase 1 cellular localization regulates the metabolic fate of glucose.
S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.
Bulk and single-cell transcriptome profiling reveal the metabolic heterogeneity in human breast cancers.
Triptolide analog LLDT-8 ameliorates psoriasis-like dermatitis in BALB/c mice via suppressing the IL-36α signaling pathway.
Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation.
Front Cell Dev Biol
BRG1 Stimulates Endothelial Derived Alarmin MRP8 to Promote Macrophage Infiltration in an Animal Model of Cardiac Hypertrophy.