S1PR3 Polyclonal antibody

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• S1PR3 Polyclonal antibody (55204-1-AP)

• 

  WB analysis using 55204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55204-1-AP (S1PR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55204-1-AP (S1PR3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

S1PR3 Polyclonal Antibody for WB, ELISA

Cat No. 55204-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

C9orf108, C9orf47, EDG 3, EDG3, Endothelial differentiation G-protein coupled receptor 3

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

55204-1-AP targets S1PR3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sphingosine-1-phosphate receptor 3
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: NM_005226
• Gene Symbol: S1PR3
• Gene ID (NCBI): 1903
• RRID: AB_3670241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99500
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S1PR3 (sphingosine-1-phosphate receptor 3), also known as S1P3 or EDG3, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P). S1P1 and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR3 has a role in the development of a stable and mature vascular system during embryonic development (PMID: 15138255).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Respir Res; S1PR3 Inhibition in alveolar epithelial cells alleviates pulmonary fibrosis by enhancing alveolar barrier function.; Authors - Mengyao Hao ; KO Validated