Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55204-1-AP targets S1PR3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | sphingosine-1-phosphate receptor 3 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | NM_005226 |
| Gene Symbol | S1PR3 |
| Gene ID (NCBI) | 1903 |
| RRID | AB_3670241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99500 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
S1PR3 (sphingosine-1-phosphate receptor 3), also known as S1P3 or EDG3, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P). S1P1 and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR3 has a role in the development of a stable and mature vascular system during embryonic development (PMID: 15138255).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for S1PR3 antibody 55204-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
S1PR3 Polyclonal antibody (55204-1-AP) has a total of 1 citation. It appears in the journal Respiratory Research (Respir Res, Impact Factor 5.7), in a 2025 study by Mengyao Hao investigating how S1PR3 inhibition in alveolar epithelial cells alleviates pulmonary fibrosis by enhancing alveolar barrier function. The antibody was used in Western blot (WB) with mouse samples. The research field associated with this citation is pulmonary fibrosis and alveolar barrier function.
| Species | Application | Title |
|---|---|---|
Respir Res S1PR3 Inhibition in alveolar epithelial cells alleviates pulmonary fibrosis by enhancing alveolar barrier function.
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