PLIN4 Polyclonal antibody

PLIN4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 55404-1-AP

Synonyms

KIAA1881, S3-12, S3 12, Perilipin 4, Adipocyte protein S3 12

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 55404-1-AP

  PC-3 cells and Jurkat cells were subjected to SDS PAGE followed by western blot with 55404-1-AP (S3-12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 55404-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55404-1-AP (S3-12 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 55404-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55404-1-AP (S3-12 antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: PC-3 cells, Jurkat
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

55404-1-AP targets PLIN4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: KIAA1881
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141 kDa
• GenBank Accession Number: NM_001080400
• Gene Symbol: KIAA1881
• Gene ID (NCBI): 729359
• RRID: AB_2881321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLIN4 (Perilipin 4) is a member of the perilipin family, a group of proteins that coat lipid droplets in adipocytes, the fatty tissue cells responsible for storing fat. The proteins in the Perilipin family are crucial regulators of lipid storage. PLIN4 expression is elevated in obese animals and humans. Plin4 is a lipid droplet protein (LDP) found predominantly in white adipose tissue (PMID: 23423172). It has lower expression in the heart, skeletal muscle, and brown adipose tissue (PMID: 17878492).

Publications

Species	Application	Title
		Nat Neurosci; TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.; Authors - Florent Laferrière
mouse	WB	J Lipid Res; Hormone-sensitive lipase protects adipose triglyceride lipase-deficient mice from lethal lipotoxic cardiomyopathy.; Authors - Mika Yamada
human	IF	Ann Clin Transl Neurol; Subsarcolemmal and cytoplasmic p62 positivity and rimmed vacuoles are distinctive for PLIN4-myopathy; Authors - Qi Wang
mouse	WB,IF	Redox Biol; Plin4 exacerbates cadmium-decreased testosterone level via inducing ferroptosis in testicular Leydig cells; Authors - Xu-Dong Zhang
goat	WB	Biol Reprod; NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells†; Authors - Hao Yu