SAC3D1 Polyclonal antibody

SAC3D1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25857-1-AP

Synonyms

HSU79266, SAC3 domain containing 1, SAC3D1, SHD1

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Validation Data Gallery

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  WB analysis using 25857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25857-1-AP (SAC3D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25857-1-AP (SAC3D1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 25857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25857-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25857-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, PC-3 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25857-1-AP is SAC3D1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAC3D1 fusion protein Ag23026
• Full Name: SAC3 domain containing 1
• Calculated Molecular Weight: 404 aa, 44 kDa
• Observed Molecular Weight: 44, 31 kDa
• GenBank Accession Number: BC007448
• Gene Symbol: SAC3D1
• Gene ID (NCBI): 29901
• RRID: AB_2918096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAC3D1, also named as SHD1 and HSU79266. SAC3D1 is widely found in the cytoplasm, cytoskeleton, microtubule, tissue center, centrosome and spindle, involved in centrosome duplication and mitotic progression. SAC3D1 is abnormally expressed in multiple types of cancer, such as colon cancer and hepatocellular carcinoma and gastric cancer. SAC3D1 may serve as a prognostic biomarker in hepatocellular carcinoma (PMID: 30353105, 32319600). SAC3D1 has 2 isoforms with the molecular mass of 44 and 31 kDa.