SAFB Polyclonal antibody

SAFB Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (2)

Cat no : 21857-1-AP

Synonyms

HAP, HET, HSP27 ERE TATA binding protein, SAF B, SAF B1, SAFB, SAFB1, scaffold attachment factor B, Scaffold attachment factor B1

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Validation Data Gallery

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  WB analysis of MCF-7 using 21857-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21857-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21857-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21857-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21857-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21857-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21857-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.

• 

  IHC staining of mouse testis using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21857-1-AP (SAFB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21857-1-AP (SAFB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.

• 

  IF Staining of A431 using 21857-1-AP

  Immunofluorescent analysis of A431 cells using 21857-1-AP (SAFB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells, MCF-7 cells, A431 cells, HCT 116 cells, COLO 320 cells
• Positive IHC detected in: mouse testis tissue, human testis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

21857-1-AP targets SAFB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAFB fusion protein Ag16451
• Full Name: scaffold attachment factor B
• Calculated Molecular Weight: 917 aa, 103 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC126219
• Gene Symbol: SAFB
• Gene ID (NCBI): 6294
• RRID: AB_2878928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport. hnRNPs also bind heterogeneous nuclear RNA (hnRNA), the transcripts produced by RNA polymerase II. SAFB (scaffold attachment factor B) is a nuclear matrix-associated protein that binds to matrix- or scaffold-associating regions (MARs or SARs) on DNA and interacts with RNA polymerase II and serine-/arginine-rich RNA processing factors (SR proteins). SAFB, also designated HAP (hnRNP A1 associated protein) and HET (HSP 27-ERE-TATA-binding protein) is a proven hnRNP protein that has a speckled distribution in the nucleus and, in response to stress agents such as heat shock, is recruited to a few, large nuclear granules, called perichromatin granules. SAFB also binds to the estrogen receptor (ER) and is expressed in several breast cancer cell lines at varying levels. Subsequently, SAFB may play a role in breast cancer by mediating cellular proliferation and division. This antibody specifically reacts whith the isoform 1 of human SAFB protein. SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SAFB is 103 kDa, but the modified SAFB is about 130-175 kDa.(PMID: 28469965, PMID: 26273616 )

Publications

Species	Application	Title
human	IF	Acta Neuropathol Commun; RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.; Authors - Mahlon Collins
human	IF	Neurobiol Dis; ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity.; Authors - Haiyan An

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Good signal in ICC, can be used to detect nuclear stress bodies (nSBs)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells

SAFB Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:21857-1-AP)

FH

Bibha (Verified Customer) (06-05-2019)

I used this antibody in human primary astrocytes Mock-infected or infected with Venezuelan equine encephalitis virus.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary astrocytes

SAFB Antibody Western Blot, validation (1:1000 dilution) in Human primary astrocytes (Cat no:21857-1-AP)