SAGE1 Polyclonal antibody

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  IHC staining of human skin cancer using 30914-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 30914-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30914-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A375 using 30914-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of U2OS using 30914-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SAGE1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 30914-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

sarcoma antigen 1, SAGE, CT14, Cancer/testis antigen 14

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Tested Applications

• Positive IHC detected in: human skin cancer tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells, U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30914-1-AP targets SAGE1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34216

  Product name: Recombinant human SAGE1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 247-387 aa of NM_018666.2

  Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH

• Full Name: sarcoma antigen 1
• Calculated Molecular Weight: 99kd
• GenBank Accession Number: NM_018666.2
• Gene Symbol: SAGE1
• Gene ID (NCBI): 55511
• RRID: AB_3669783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NXZ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).