SALL1 Polyclonal antibody

• 

  IHC staining of human placenta using 27761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 27761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 27761-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 27761-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 27761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 27761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 27761-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SALL1 antibody (27761-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 27761-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SALL1 antibody (27761-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SALL1 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 27761-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

HSAL1, Sal 1, Sal like protein 1, SAL1, TBS

---

Tested Applications

• Positive IHC detected in: mouse testis tissue, human placenta tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27761-1-AP targets SALL1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SALL1 fusion protein Ag26766
• Full Name: sal-like 1 (Drosophila)
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_001127892
• Gene Symbol: SALL1
• Gene ID (NCBI): 6299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NSC2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SALL1(Sal-like protein 1), also known as SAL1. It is located in the cytoplasm and nucleus. The protein is mainly expressed in the kidney. Transcriptional repressor involved in organogenesis. The protein plays an essential role in ureteric bud invasion during kidney development. The molecular weight of SALL1 is 140 kDa.