SALL2 Polyclonal antibody

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  WB analysis of Jurkat using 23114-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23114-1-AP (SALL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23114-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23114-1-AP (SALL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SALL2 Polyclonal Antibody for WB, ELISA

Cat No. 23114-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HSAL2, KIAA0360, p150(Sal2), Sal 2, sal like 2 (Drosophila), Sal like protein 2, SAL2, SALL2, Zinc finger protein SALL2, Zinc finger protein Spalt 2, ZNF795

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23114-1-AP targets SALL2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19427

  Product name: Recombinant human SALL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 753-1007 aa of BC136528

  Sequence: SQQPSPEEELSEEEEEEDEEEEEDVTDEDSLAGRGSESGGEKAISVRGDSEEASGAEEEVGTVAAAATAGKEMDSNEKTTQQSSLPPPPPPDSLDQPQPMEQGSSGVLGGKEEGGKPERSSSPASALTPEGEATSVTLVEELSLQEAMRKEPGESSSRKACEVCGQAFPSQAALEEHQKTHPKEGPLFTCVFCRQGFLERATLKKHMLLAHHQVQPFAPHGPQNIAALSLVPGCSPSITSTGLSPFPRKDDPTIP

• Full Name: sal-like 2 (Drosophila)
• Calculated Molecular Weight: 1007 aa, 105 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC136528
• Gene Symbol: SALL2
• Gene ID (NCBI): 6297
• RRID: AB_2879212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9Y467
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sall2 (SALL2, sal-like 2, HSAL2, p150(Sal2)) are mammalian homologs of the Drosophila region-specific home-otic gene spalt (sal), which encodes a zinc finger-containing transcription regulator. Mammalian Sall1 may mediate higher order chromatin structure and may be a component of a distinct heterochromatin-dependent silencing process. Sall2 is a p53-independent regulator of p21 and BAX, and can function in some cell types as a regulator of cell growth and survival. Human Sall2 is present in brain, heart, kidney and pancreas. This antibody is specific to the SALL2 protein.