SALL4 Monoclonal antibody, PBS Only

• 

  WB analysis of K-562 using 66236-1-Ig (same clone as 66236-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66236-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66236-1-Ig (same clone as 66236-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66236-1-PBS in a different storage buffer formulation.

SALL4 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66236-1-PBS

Clone No.2B5B2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

2B5B2, DRRS, HSAL4, Sal like protein 4, Sal-like protein 4

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Product Information

66236-1-PBS targets SALL4 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17480

  Product name: Recombinant human SALL4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC111714

  Sequence: MSRRKQAKPQHINSEEDQGEQQPQQQTPEFADAAPAAPAAGELGAPVNHPGNDEVASEDEATVKRLRREETHVCEKCCAEFFSISEFLEHKKNCTKNPPVLIMNDSEGPVPSEDFSGAVLSHQPTSPGSKDCHRENGGSSEDMKEKPDAESVVYLKTETALPPTPQDISYLAKGKVANTNVTLQALRGTKVAVNQRSADALPAPVPGANSIPWVLEQILCLQQQQLQQIQLTEQIRIQVNMWASHALHSSGAGADTLKTLGSHMSQQVSAAVALLSQKAGSQGLSLDALKQAKLPHANIPSATSSLSPGLAPFTLKPDGTRVLPNVMSRLPSALLPQAPGSVLFQSPFST

• Full Name: sal-like 4 (Drosophila)
• Calculated Molecular Weight: 1053 aa, 112 kDa
• Observed Molecular Weight: 165 kDa
• GenBank Accession Number: BC111714
• Gene Symbol: SALL4
• Gene ID (NCBI): 57167
• RRID: AB_2881625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UJQ4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SALL4, also named as Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta
-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.