SAMD8 Polyclonal antibody

SAMD8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26815-1-AP

Synonyms

SAMD8, SMSr

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Validation Data Gallery

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  WB analysis of mouse heart using 26815-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26815-1-AP (SAMD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 26815-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26815-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 26815-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 26815-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

26815-1-AP targets SAMD8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAMD8 fusion protein Ag25310
• Full Name: sterile alpha motif domain containing 8
• Observed Molecular Weight: 38 kDa, 45 kDa
• GenBank Accession Number: BC146556
• Gene Symbol: SAMD8
• Gene ID (NCBI): 142891
• RRID: AB_2880644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF	J Biol Chem; Sphingomyelin synthase-related protein SMSr is a phosphatidylethanolamine phospholipase C that promotes nonalcoholic fatty liver disease; Authors - Yeun-Po Chiang