Tested Applications
| Positive WB detected in | rat kidney tissue, COLO 320 cells |
| Positive IHC detected in | mouse spleen tissue, human brain tissue, rat ovary tissue, human ovary cancer tissue, rat lung tissue, human breast cancer tissue, human colon cancer tissue, mouse lung tissue, human placenta tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, 3T3-L1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25173-1-AP targets SAMD9L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18541 Product name: Recombinant human SAMD9L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC127118 Sequence: MSKQVSLPEMIKDWTKEHVKKWVNEDLKINEQYGQILLSEEVTGLVLQELTEKDLVEMGLPWGPALLIKRSYNKLNSKSPESDNHDPGQLDNSKPSKTEHQKNPKHTKKEEENSMSSNIDYDPREIRDIKQEESILMKENVLDEVANAKHKKKGKLKPEQLTCMPYPFDQFHDSHRYIEHYTLQPETGALNLI Predict reactive species |
| Full Name | sterile alpha motif domain containing 9-like |
| Calculated Molecular Weight | 1584 aa, 185 kDa |
| Observed Molecular Weight | 160-180 kDa |
| GenBank Accession Number | BC127118 |
| Gene Symbol | SAMD9L |
| Gene ID (NCBI) | 219285 |
| RRID | AB_2879940 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IVG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sterile alpha motif domain-containing protein 9-like(SAMD9L), also named C7orf6, DRIF2, KIAA2005, and UEF, is a cytoplasmic protein that acts as a tumor suppressor but also plays a key role in cell proliferation and the innate immune response to viral infection. SAMD9L protein is an endosome fusion facilitator that causes myeloid malignancies in mice similar to human diseases with monosomy 7. The SAMD9L protein also appears to play an important role in the brain, particularly the part of the brain that coordinates movement (the cerebellum). SAMD9L has two isoforms with MW 185 kDa and 47 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| IHC protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| WB protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SAMD9L Polyclonal antibody (Cat# 25173-1-AP) has 12 citations. These appear in journals including Nature Medicine, Nature Communications, Nature Ecology & Evolution, PLoS Biology, PLoS Pathogens, Journal of Inflammation Research, Brain Communications, Experimental Cell Research, Immunobiology, Experimental Hematology, and bioRxiv. Research fields span antiviral immunity, viral restriction factors, clonal hematopoiesis, neurodegeneration (SCA49), Sjögren's syndrome, and cell cycle regulation.
| Species | Application | Title |
|---|---|---|
Nat Med Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes. | ||
Nat Commun SAMD9 senses cytosolic double-stranded nucleic acids in epithelial and mesenchymal cells to induce antiviral immunity | ||
Nat Ecol Evol Evolutionary characterization of antiviral SAMD9/9L across kingdoms supports ancient convergence and lineage-specific adaptations. | ||
PLoS Biol SAMD9L acts as an antiviral factor against HIV-1 and primate lentiviruses by restricting viral and cellular translation | ||
PLoS Pathog A paralogous pair of mammalian host restriction factors form a critical host barrier against poxvirus infection.
| ||
PLoS Pathog SAMD9L inhibits flavivirus translation independently of its capacity to trigger innate immune response.
|
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used it for Western Blot at a 1:5000 dilution on cell lines and reported that it performed very well in recognizing SAMD9L from human, monkey, and golden Syrian hamster samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaopeng (Verified Customer) (09-02-2025) | very good to recognize human, monkey, and golden Syrian hamster samd9l by western blot.
|
