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  • KD/KO Validated

Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20824-1-AP

Synonyms

CGI 51, OMP85, Sam50, SAMM50, TOB55, TRG 3, TRG3, YNL026W



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, A375 cells, mouse ovary tissue, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20824-1-AP is Sam50 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sam50 fusion protein Ag14799
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Calculated molecular weight 469 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007830
Gene symbol SAMM50
Gene ID (NCBI) 25813
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

Protocols

Product Specific Protocols
WB protocol for Sam50 antibody 20824-1-APDownload protocol
IHC protocol for Sam50 antibody 20824-1-APDownload protocol
IF protocol for Sam50 antibody 20824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

Authors - Virginia Guarani
humanWB

Cell Rep

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

Authors - Timothy D Martin
  • KD Validated
ratWB

Free Radic Biol Med

Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy.

Authors - Run-Qing Xue
mouseWB

Free Radic Biol Med

PMID: 30633969

Authors - Run-Qing Xue