Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, mouse heart tissur, rat heart tissue |
| Positive IHC detected in | human heart tissue, human liver cancer tissue, mouse adrenal gland tissue, rat kidney tissue, rat adrenal gland tissue, human placenta tissue, mouse kidney tissue, human ovary cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28679-1-AP targets Sam50 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29152 Product name: Recombinant human SAMM50 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC007830 Sequence: MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPW Predict reactive species |
| Full Name | sorting and assembly machinery component 50 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 469 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC007830 |
| Gene Symbol | Sam50 |
| Gene ID (NCBI) | 25813 |
| RRID | AB_2881192 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y512 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Sam50 antibody 28679-1-AP | Download protocol |
| IHC protocol for Sam50 antibody 28679-1-AP | Download protocol |
| WB protocol for Sam50 antibody 28679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Sam50 Polyclonal antibody (Cat# 28679-1-AP) has 11 citations published in journals including Brain, Dev Cell, J Nanobiotechnology, Int J Biol Sci, Cell Signal, Cancer Res Commun, J Cell Sci, Front Cardiovasc Med, Cells, Biol Proced Online, and bioRxiv. Research fields span mitochondrial biology, hepatology (MASH), cardiology, nephrology, oncology (HCC, lung carcinoma), neurology, ferroptosis, and exercise physiology.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology UBQLN1 Inhibition reduces MASH progression through downregulating SIKE/p38 MAPK pathway in hepatocyte. | ||
Brain Mitochondrial damage is associated with an early immune response in inclusion body myositis. | ||
Int J Biol Sci Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients | ||
Cells Heart-Specific and Conditional Deletion of the Immt Gene Reveals Its Role in Regulating Mitochondrial Structure and Total Heart Metabolism. | ||
Biol Proced Online High Expression of SAMM50 Indicates Poor Clinical Prognosis in Hepatocellular Carcinoma and Represents a Potential Novel Biomarker. |
Reviews
What customers say
One verified customer rated this product 5 stars, reporting excellent performance in Western Blot at a 1:8000 dilution. The reviewer expressed satisfaction with both the quality and cost-effectiveness of the product, noting that the WB results matched expectations and previously published reports. Overall, the feedback is positive, confirming reliable and consistent performance for its intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yelena (Verified Customer) (01-20-2026) | Works well. Happy with the quality and cost of this product, the WB looked as advertised and previously reported.
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