SAP30 Polyclonal antibody

SAP30 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27679-1-AP

Synonyms

SAP30

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Validation Data Gallery

• 

  WB analysis of A549 using 27679-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 27679-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 27679-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HL-60 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

27679-1-AP targets SAP30 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAP30 fusion protein Ag26516
• Full Name: Sin3A-associated protein, 30kDa
• Calculated Molecular Weight: 220 aa, 23 kDa
• Observed Molecular Weight: 23-28 kDa
• GenBank Accession Number: BC016757
• Gene Symbol: SAP30
• Gene ID (NCBI): 8819
• RRID: AB_2880945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAP30, also named as Sin3 corepressor complex subunit SAP30, is a 220 amino acid protein, which is expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes.

Publications

Species	Application	Title
	IF	Development; Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures.; Authors - Sandra Ruiz García
human	WB	Cell Rep; SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns; Authors - Kazuhiro Fukumura