SAPS3 Polyclonal antibody

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  WB analysis using 16944-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16944-1-AP (SAPS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16944-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16944-1-AP (SAPS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 16944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16944-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16944-1-AP (SAPS3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

SAPS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16944-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SAPLa, SAPL, PPP6R3, PP6R3, KIAA1558

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

16944-1-AP targets SAPS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10555

  Product name: Recombinant human SAPS3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-336 aa of BC020953

  Sequence: VRERWETFCTSSLGETNKRNTVDLFGFNDEKFADQDDIGNVSFDRVSDINFTLNTNESGNIALFEACCKERIQQFDDGGSDEEDIWEEKHIAFTPESQRRSSSGSTDSEESTDSEEEDGAKQDLFEPSSANTEDKMEVDLSEPPNWSANFDVPMETTHGAPLDSVGSDVWSTEEPMPTKETGWASFSEFTSSLSTKDSLRSNSPVEMETSTEPMDPLTPSAAALAVQPEAAGSVAMEASSDGEEDAESTDKVTETVMNGGMKETLSLTVDAKTETAVFKSEEGKLSTSQDAACKDAEECPETAEAKCAAPRPPSSSPEQRTGQPSAPGDTSVNGPV

• Full Name: SAPS domain family, member 3
• Calculated Molecular Weight: 873 aa, 98 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC020953
• Gene Symbol: SAPS3
• Gene ID (NCBI): 55291
• RRID: AB_2183826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5H9R7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Biol Chem; A conserved acetylation switch enables pharmacological control of tubby-like protein stability.; Authors - Evan M Kerek
human	IP	Cell Death Dis; PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death; Authors - Guowei Wu
	WB	BMC Biol; The protein phosphatase PP6 promotes RIPK1-dependent PANoptosis; Authors - Ratnakar R Bynigeri