SAR1A Polyclonal antibody

• Featured Product
• KD/KO Validated

SAR1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15350-1-AP

Synonyms

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1

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Validation Data Gallery

• 

  WB analysis of mouse liver using 15350-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 15350-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 15350-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15350-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15350-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, HT-1080 cells
• Positive IHC detected in: human hepatocirrhosis tissue, human liver tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

15350-1-AP targets SAR1A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAR1A fusion protein Ag7596
• Full Name: SAR1 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC003658
• Gene Symbol: SAR1A
• Gene ID (NCBI): 56681
• RRID: AB_10667406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human,mouse	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB	Proc Natl Acad Sci U S A; Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.; Authors - Liling Niu
rat	WB	J Biol Chem; The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.; Authors - Lee G D Fryer
human,mouse	IHC,WB	Clin Epigenetics; Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma.; Authors - Hongxia Li ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.; Authors - Yuri Urai