SAR1B Polyclonal antibody

SAR1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15453-1-AP

Synonyms

ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB

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Validation Data Gallery

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  WB analysis using 15453-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15453-1-AP (SAR1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15453-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15453-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15453-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15453-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15453-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15453-1-AP (SAR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15453-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15453-1-AP (SAR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: L02 cells, mouse brain tissue, human liver tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15453-1-AP targets SAR1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAR1B fusion protein Ag7718
• Full Name: SAR1 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC002847
• Gene Symbol: SAR1B
• Gene ID (NCBI): 51128
• RRID: AB_2183985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex.