SARS Polyclonal antibody

SARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15162-1-AP

Synonyms

Seryl-tRNA(Ser/Sec) synthetase, Seryl-tRNA synthetase, SERS, SERRS, Serine--tRNA ligase, cytoplasmic

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Validation Data Gallery

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  WB analysis of HEK-293 using 15162-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15162-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15162-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15162-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15162-1-AP (SARS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 15162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15162-1-AP (SARS antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15162-1-AP

  Immunofluorescent analysis of HepG2 cells using 15162-1-AP (SARS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15162-1-AP targets SARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SARS fusion protein Ag7270
• Full Name: seryl-tRNA synthetase
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58-64 kDa
• GenBank Accession Number: BC000716
• Gene Symbol: SARS
• Gene ID (NCBI): 6301
• RRID: AB_2184009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SARS(Serine--tRNA ligase, cytoplasmic) Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).The deduced 514-amino acid protein has a calculated molecular mass of about 59 kD.And it can form a dimer of 120KDa(PMID:2180700).It is detected a band of 65KDa in MEL cell lines(PMID:1918033).

Publications

Species	Application	Title
human	WB	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu