Tested Applications
| Positive ELISA detected in | Recombinant protein, N/A |
Recommended dilution
| Application | Dilution |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29264-1-AP targets SARS-COV-2 NSP12 in WB, IF, ELISA applications and shows reactivity with Virus samples.
| Tested Reactivity | Virus |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30931 Product name: Recombinant sars-cov-2 NSP12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of YP_009725307 Sequence: SADAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAG Predict reactive species |
| Full Name | ORF1ab |
| GenBank Accession Number | YP_009725307 |
| Gene Symbol | SARS-COV-2 NSP8 |
| Gene ID (NCBI) | 43740578 |
| RRID | AB_2918262 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
The SARS-CoV-2 NSP12 Polyclonal antibody (Cat# 29264-1-AP) has 1 citation. It appears in the Journal of Cell Biology (2024), where it was used for WB and IF to study SARS-CoV-2 NSP3/4-mediated replication organelle formation and RNA polymerase NSP12 recruitment in human samples. The research field is virology, specifically coronavirus replication biology.
| Species | Application | Title |
|---|---|---|
J Cell Biol SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12 |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody at a 1:1000 dilution for Western Blot on Vero cell lysates infected with SARS-CoV-2 and reported excellent performance with no background signal. Overall, the feedback highlights strong specificity and clean results in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marie-Lise (Verified Customer) (12-15-2021) | very good antibody for WB with a 1/1000 dilution on Vero lysates infected with SARS-CoV-2 no background
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