Sars-cov-2 SARS-COV-2 NSP5 Polyclonal antibody, PBS Only

• 

  IF Staining of HEK-293T using 29286-1-AP (same clone as 29286-1-PBS)

  Immunofluorescent analysis of 4% PFA fixed HEK293T cells overexpressing ACE2 and TMPRSS using NSP5 antibody (29286-1-AP, green) at dilution of 1:200. Cells were infected with virus (dsRNA, in red, MOI 0.01, 48hpi). The images were credited by Dr. Irene Chen, Gladstone Institute. This data was developed using the same antibody clone with 29286-1-PBS in a different storage buffer formulation.

• 

  ELISA experiment using 29286-1-AP (same clone as 29286-1-PBS)

  SARS-CoV-2 Envelope protein Antibody (29286-1-AP) tested by ELISA. SARS-COV-2 NSP5 protein was coated onto microtiter plates at 1 µg/well and then incubated with a dilution series of SARS-COV-2 NSP5 protein Antibody (29286-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. This data was developed using the same antibody clone with 29286-1-PBS in a different storage buffer formulation.

SARS-COV-2 NSP5 Polyclonal Antibody for IF/ICC, ELISA, Indirect ELISA

Cat No. 29286-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

IF/ICC, ELISA, Indirect ELISA

Synonyms

---

Product Information

29286-1-PBS targets SARS-COV-2 NSP5 in IF/ICC, ELISA, Indirect ELISA applications and shows reactivity with virus samples.

• Tested Reactivity: virus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30934

  Product name: Recombinant sars-cov-2 NSP5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-306 aa of YP_009742612

  Sequence: SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ

• Full Name: ORF1ab
• GenBank Accession Number: YP_009742612
• Gene Symbol: SARS-COV-2 NSP8
• Gene ID (NCBI): 43740578
• RRID: AB_2918269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.