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SART1 Polyclonal antibody

SART1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22675-1-AP

Synonyms

Ara1, Hom s 1, HOMS1, hSART 1, hSnu66, SART 1, SART1, SART1259, SNRNP110, Snu66, SNU66 homolog



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22675-1-AP is SART1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SART1 fusion protein Ag18478
Full Name squamous cell carcinoma antigen recognized by T cells
Calculated Molecular Weight 800 aa, 90 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC001058
Gene Symbol SART1
Gene ID (NCBI) 9092
RRIDAB_2879148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Squamous cell carcinoma antigen recognized by T cells (SART1), also known as U4/U6.U5 tri-snRNP-associated 110 kDa protein, is a ubiquitously expressed protein that is involved in mRNA splicing and the regulation of cell proliferation. It has been identified as regulator of c-FLIP and drug-induced activation of caspase 8. The SART1 gene encodes two proteins, the SART1(800) protein is located in the nucleus of the majority of proliferating cells, while the SART1(259) protein is located in the cytosol of epithelial cancers. Genetic variation in the SART1 gene may be associated with breast cancer development. This antibody recognizes the endogenous 800-amino acid SART1, which migrates with an apparent molecular mass of 110 kDa in SDS-PAGE. (PMID: 22027693; 9449708; 19377877; 11350945)

Protocols

Product Specific Protocols
WB protocol for SART1 antibody 22675-1-APDownload protocol
IHC protocol for SART1 antibody 22675-1-APDownload protocol
IF protocol for SART1 antibody 22675-1-APDownload protocol
IP protocol for SART1 antibody 22675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

Theranostics

Suppressing Sart1 to modulate macrophage polarization by siRNA-loaded liposomes: a promising therapeutic strategy for pulmonary fibrosis.

Authors - Ting Pan
  • KD Validated