SART1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SART1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22675-1-AP

Synonyms

hSnu66, hSART-1, hSART 1, HOMS1, Hom s 1

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Validation Data Gallery

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  WB analysis of HeLa using 22675-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22675-1-AP (SART1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22675-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22675-1-AP (SART1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22675-1-AP

  IP result of anti-SART1 (IP:22675-1-AP, 4ug; Detection:22675-1-AP 1:6000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of mouse testis using 22675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22675-1-AP

  Immunofluorescent analysis of HepG2 cells, using SART1 antibody 22675-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

22675-1-AP targets SART1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SART1 fusion protein Ag18478
• Full Name: squamous cell carcinoma antigen recognized by T cells
• Calculated Molecular Weight: 800 aa, 90 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC001058
• Gene Symbol: SART1
• Gene ID (NCBI): 9092
• RRID: AB_2879148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Squamous cell carcinoma antigen recognized by T cells (SART1), also known as U4/U6.U5 tri-snRNP-associated 110 kDa protein, is a ubiquitously expressed protein that is involved in mRNA splicing and the regulation of cell proliferation. It has been identified as regulator of c-FLIP and drug-induced activation of caspase 8. The SART1 gene encodes two proteins, the SART1(800) protein is located in the nucleus of the majority of proliferating cells, while the SART1(259) protein is located in the cytosol of epithelial cancers. Genetic variation in the SART1 gene may be associated with breast cancer development. This antibody recognizes the endogenous 800-amino acid SART1, which migrates with an apparent molecular mass of 110 kDa in SDS-PAGE. (PMID: 22027693; 9449708; 19377877; 11350945)

Publications

Species	Application	Title
mouse	IF,WB	Theranostics; Suppressing Sart1 to modulate macrophage polarization by siRNA-loaded liposomes: a promising therapeutic strategy for pulmonary fibrosis.; Authors - Ting Pan ; KD Validated
human	WB	Cell Rep; SART3 reads methylarginine-marked glycine- and arginine-rich motifs; Authors - Yalong Wang