SAV1 Polyclonal antibody

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• SAV1 Polyclonal antibody (32277-1-AP)

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  WB analysis using 32277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32277-1-AP (SAV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 32277-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 32277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 32277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 32277-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 32277-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SAV1 antibody (32277-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SAV1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 32277-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

45 kDa WW domain protein, hWW45, Protein salvador homolog 1, SAV

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Tested Applications

• Positive WB detected in: HT-29 cells, HUVEC cells, U2OS cells
• Positive IHC detected in: mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32277-1-AP targets SAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36278

  Product name: Recombinant human SAV1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 208-383 aa of BC020537

  Sequence: DWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF

• Full Name: salvador homolog 1 (Drosophila)
• Calculated Molecular Weight: 383 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC020537
• Gene Symbol: SAV1
• Gene ID (NCBI): 60485
• RRID: AB_3742564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H4B6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Salvador family WW domain‐containing 1 (SAV1, also known as WW45) is a human homologue of Salvador that contains two protein‐protein interaction modules known as WW domains and acts as a scaffolding protein (PMID: 28618450). SAV1 belongs to the core kinase components of Hippo signalling pathway for efficient MST1/2 activation. SAV1 represses the growth of colorectal cancer cells by inactivating Akt‐mTOR signalling in a YAP‐dependent manner (PMID: 29063833, 22185343).