SAV1 Monoclonal antibody

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  WB analysis using 60903-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60903-1-Ig (SAV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 60903-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60903-1-Ig (SAV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60903-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60903-1-Ig (SAV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SAV1 Monoclonal Antibody for WB, ELISA

Cat No. 60903-1-Ig

Clone No.1G1H11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

45 kDa WW domain protein, hWW45, Protein salvador homolog 1, SAV

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Tested Applications

• Positive WB detected in: LoVo cells, HSC-T6 cells, NIH/3T3 cells, A431 cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60903-1-Ig targets SAV1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3441

  Product name: Recombinant human SAV1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-376 aa of BC020537

  Sequence: MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDICLPDSSPNAFSTSGDVVSRNQSFLRTPIQRTPHEIMRRESNRLSAPSYLARSLADVPREYGSSQSFVTEVSFAVENGDSGSRYYYSDNFFDGQRKRPLGDRAHEDYRYYEYNHDLFQRMPQNQGRHASGIGRVAATSLGNLTNHGSEDLPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYA

• Full Name: salvador homolog 1 (Drosophila)
• Calculated Molecular Weight: 383 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC020537
• Gene Symbol: SAV1
• Gene ID (NCBI): 60485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9H4B6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Salvador family WW domain‐containing 1 (SAV1, also known as WW45) is a human homologue of Salvador that contains two protein‐protein interaction modules known as WW domains and acts as a scaffolding protein (PMID: 28618450). SAV1 belongs to the core kinase components of Hippo signalling pathway for efficient MST1/2 activation. SAV1 represses the growth of colorectal cancer cells by inactivating Akt‐mTOR signalling in a YAP‐dependent manner (PMID: 29063833, 22185343).