SBNO1 Polyclonal antibody, PBS Only

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• SBNO1 Polyclonal antibody, PBS Only (25359-1-PBS)

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  WB analysis using 25359-1-AP (same clone as 25359-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25359-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 25359-1-AP (same clone as 25359-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25359-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 25359-1-AP (same clone as 25359-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25359-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 25359-1-AP (same clone as 25359-1-PBS)

  IP result of anti-SBNO1 (IP:25359-1-AP, 4ug; Detection:25359-1-AP 1:3000) with Jurkat cells lysate 1400 ug. This data was developed using the same antibody clone with 25359-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 25359-1-AP (same clone as 25359-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SBNO1 antibody (25359-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 25359-1-PBS in a different storage buffer formulation.

SBNO1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 25359-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

25359-1-PBS targets SBNO1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19005

  Product name: Recombinant human SBNO1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-300 aa of BC133704

  Sequence: MVEPGQDLLLAALSESGISPNDLFDIDGGDAGLATPMPTPSVQQSVPLSALELGLETEAAVPVKQEPETVPTPALLNVRQQPPSTTTFVLNQINHLPPLGSTIVMTKTPPVTTNRQTITLTKFIQTTASTRPSVSAPTVRNAMTSAPSKDQVQLKDLLKNNSLNELMKLKPPANIAQPVATAATDVSNGTVKKESSNKEGARMWINDMKMRSFSPTMKVPVVKEDDEPEEEDEEEMGHAETYAEYMPIKLKIGLRHPDAVVETSSLSSVTPPDVWYKTSISEETIDNGWLSALQLEAITY

• Full Name: strawberry notch homolog 1 (Drosophila)
• Calculated Molecular Weight: 1393 aa, 154 kDa
• Observed Molecular Weight: 145-154 kDa
• GenBank Accession Number: BC133704
• Gene Symbol: SBNO1
• Gene ID (NCBI): 55206
• RRID: AB_2880042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A3KN83
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SBNO1, also named as Monocyte protein 3 or MOP3, is a 1393 amino acid protein, which belongs to the SBNO family. SBNO1 play an important role in development of the vertebrate central nervous system. SBNO1 has four isoforms with MW 154 kDa and 80 kDa.