SC4MOL Polyclonal antibody

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  WB analysis using 31159-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31159-1-AP (SC4MOL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SC4MOL Polyclonal Antibody for WB, ELISA

Cat No. 31159-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

C 4 methylsterol oxidase, DESP4, ERG25, Methylsterol monooxygenase, SC4MOL, sterol C4 methyl oxidase like

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Tested Applications

• Positive WB detected in: SW 1990 cells, mouse adipose tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31159-1-AP targets SC4MOL in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SC4MOL fusion protein Ag34758
• Full Name: sterol-C4-methyl oxidase-like
• Observed Molecular Weight: 34-36 kDa
• GenBank Accession Number: BC107879
• Gene Symbol: SC4MOL
• Gene ID (NCBI): 6307
• RRID: AB_3669874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q15800
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SC4MOL(sterol C4-methyl oxidase-like), an intermediate enzyme in the cholesterol biosynthetic pathway, was included in the library based on its representation in a high confidence GEO transcriptional profiling dataset as a transcript that rapidly undergoes significant expression change in response to stimulation or inhibition of EGFR. Among the validated hits that increased cell killing by common EGFR antagonists, SC4MOL was consistently one of the most effective modulators. (PMID: 23125191)