SCAI Polyclonal antibody

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• SCAI Polyclonal antibody (33449-1-AP)

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  WB analysis using 33449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33449-1-AP (SCAI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

SCAI Polyclonal Antibody for WB, ELISA

Cat No. 33449-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C9orf126, Protein SCAI, Suppressor of cancer cell invasion protein

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33449-1-AP targets SCAI in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38169

  Product name: Recombinant human C9orf126 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 192-414 aa of NM_001144877.2

  Sequence: LNKMDVVKDLVKELSDEIEDYTHRFNTEDQVEWNLVLQEVAAFIEADPVMVLNDDNTIVITSNRLAETGAPLLEQGMIVGQLSLADALIIGNCNNQVKFSELTVDMFRMLQALEREPMNLASQMNKPGMQESADKPTRRENPHKYLLYKPTFSQLYTFLAASFKELPANSVLLIYLSATGVFPTGRSDSEGPYDFGGVLTNSNRDIINGDAIHKRNQSHKEMH

• Full Name: chromosome 9 open reading frame 126
• Calculated Molecular Weight: 70kDa，606aa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_001144877.2
• Gene Symbol: C9orf126
• Gene ID (NCBI): 286205
• RRID: AB_3742975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8N9R8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCAI (suppressor of cancer cell invasion) is a conserved protein that regulates invasive cell migration through three-dimensional matrices. SCAI acts on the RhoA-Dia1 signal transduction pathway and localizes in the nucleus, where it binds and inhibits the myocardin-related transcription factor MAL by forming a ternary complex with serum response factor (SRF). Genome-wide expression analysis surprisingly reveals that one of the strongest upregulated genes after suppression of SCAI is beta1-integrin. Decreased levels of SCAI are tightly correlated with increased invasive cell migration, and SCAI is downregulated in several human tumours. Functional analysis of the beta1-integrin gene strongly argues that SCAI is a novel transcriptional cofactor that controls gene expression downstream of Dia1 to dictate changes in cell invasive behaviour (PMID: 19350017).