SCAMP1 Polyclonal antibody

SCAMP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15327-1-AP

Synonyms

SCAMP, SCAMP1, SCAMP37



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse pancreas tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15327-1-AP targets SCAMP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCAMP1 fusion protein Ag3258
Full Name secretory carrier membrane protein 1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC034048
Gene Symbol SCAMP1
Gene ID (NCBI) 9522
RRIDAB_2184766
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SCAMP1 antibody 15327-1-APDownload protocol
IHC protocol for SCAMP1 antibody 15327-1-APDownload protocol
IP protocol for SCAMP1 antibody 15327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Int J Mol Sci

Multiomics Analysis of Endocytosis upon HBV Infection and Identification of SCAMP1 as a Novel Host Restriction Factor against HBV Replication.

Authors - Tanzeel Yousaf
humanIHC

Cell Death Dis

MTSS1 and SCAMP1 cooperate to prevent invasion in breast cancer.

Authors - Jayakumar Vadakekolathu
humanWB

Aging (Albany NY)

Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.

Authors - Feiyu Mao
humanWB,IHC

J Cancer

SCAMP1 silencing inhibits proliferation by attenuating multiple pro-survival signaling pathways in gastric cancer

Authors - Gang Ma