Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse pancreas tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15327-1-AP targets SCAMP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3258 Product name: Recombinant human SCAMP1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-338 aa of BC034048 Sequence: MSDFDSNPFADPDLNNPFKDPSVTQVTRNVPPGLDEYNPFSDSRTPPPGGVKMPNVPNTQPAIMKPTEEHPAYTQIAKEHALAQAELLKRQEELERKAAELDRREREMQNLSQHGRKNNWPPLPSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYLWMFHAVTLFLNIFGCLAWFCVDSARAVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAVHVLQAAGFHNWGNCGWISSLTGLNQNIPVGIMMIIIAALFTASAVISLVMFKKVHGLYRTTGASFEKAQQEFATGVMSNKTVQTAAANAASTAASSAAQNAFKGNQI Predict reactive species |
| Full Name | secretory carrier membrane protein 1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC034048 |
| Gene Symbol | SCAMP1 |
| Gene ID (NCBI) | 9522 |
| RRID | AB_2184766 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SCAMP1 antibody 15327-1-AP | Download protocol |
| IP protocol for SCAMP1 antibody 15327-1-AP | Download protocol |
| WB protocol for SCAMP1 antibody 15327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SCAMP1 antibody (catalog number 15327-1-AP) has 5 total citations. These appear in journals including Cell Death & Disease, Cell Communication and Signaling, Aging, International Journal of Molecular Sciences, and Journal of Cancer. The associated research fields span breast cancer invasion, renal cancer angiogenesis, pancreatic adenocarcinoma prognosis, HBV infection and host restriction, and gastric cancer proliferation and signaling pathways.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal TCOF1 affects Golgi secretory pathway contributing to the angiogenesis in renal cancer. | ||
Aging (Albany NY) Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma. | ||
Int J Mol Sci Multiomics Analysis of Endocytosis upon HBV Infection and Identification of SCAMP1 as a Novel Host Restriction Factor against HBV Replication. | ||
J Cancer SCAMP1 silencing inhibits proliferation by attenuating multiple pro-survival signaling pathways in gastric cancer |
Reviews
What customers say
The single review rates this antibody 5 stars for Western Blot. The user reports it works perfectly, producing a clear single band of the expected size across nearly all tested tissues and cell lines, including mouse heart, lung, liver, forebrain, primary cerebellar neurons, Neuro2A, and HEK293T. It recognizes both mouse and human Scamp1. The dilution used was 1:1000. Overall, the feedback highlights strong specificity, consistent performance, and reliable cross-reactivity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Olga (Verified Customer) (01-17-2025) | Works perfectly in Western. Gives a clear single band of the corresponding size in almost all tissues and cell lines tested. Recognizes mouse and human Scamp1.
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