SCARA5 Polyclonal antibody

SCARA5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55390-1-AP

Synonyms

NET33, SCARA5, Scavenger receptor hlg, Tesr

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Validation Data Gallery

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  WB analysis of mouse liver using 55390-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55390-1-AP (SCARA5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55390-1-AP targets SCARA5 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: scavenger receptor class A, member 5 (putative)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: NM_173833
• Gene Symbol: SCARA5
• Gene ID (NCBI): 286133
• RRID: AB_11182487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCARA5 belongs to the SCARA5 family. It is a ferritin receptor that mediates non-transferrin-dependent delivery of iron. SCARA5 mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. SCARA5 binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1). This antibody can recognize isoform 1,2 and 3 of SCARA5.

Publications

Species	Application	Title
mouse	WB	J Biomater Appl; Prussian blue-modified ferritin nanoparticles for effective tumor chemo-photothermal combination therapy via enhancing reactive oxygen species production.; Authors - Hao Li