SCARF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SCARF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13702-1-AP

Synonyms

Acetyl LDL receptor, KIAA0149, SCARF1, SREC, SREC-I

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Validation Data Gallery

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  WB analysis using 13702-1-AP

  Boiled mouse lung tissue and unboiled mouse lung tissue were subjected to SDS PAGE followed by western blot with 13702-1-AP (SCARF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse lung tissue, unboiled mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

13702-1-AP targets SCARF1 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCARF1 fusion protein Ag4621
• Full Name: scavenger receptor class F, member 1
• Calculated Molecular Weight: 830 aa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC039735
• Gene Symbol: SCARF1
• Gene ID (NCBI): 8578
• RRID: AB_2182983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Scavenger receptor class F member 1 (SCARF1), also known as scavenger receptor expressed by endothelial cells (SREC)-I, belongs to the scavenger receptor (SR) superfamily. SCARF1 has been shown to bind modified low density lipoproteins (LDLs), including acetylated (Ac-) and oxidized (ox-) LDLs (PMID: 29725698; PMID: 15145948). In addition to binding and internalizing a diverse range of endogenous proteins, SCARF1 also binds a wide array of viral, fungal, and bacterial antigens (PMID: 29242513). SCARF1 has a calculated molecular weight of 87 kDa, with a molecular weight of 140-160 kDa due to N-glycosylation (PMID: 22279061; PMID: 15145948). SCARF1 can also exist as a dimer (~ 180 kDa) and a truncated soluble form (~ 60 kDa) (PMID: 29242513; PMID: 29725698).

Publications

Species	Application	Title
mouse	IF	Nat Immunol; The scavenger receptor SCARF1 mediates the clearance of apoptotic cells and prevents autoimmunity.; Authors - Ramirez-Ortiz Zaida G ZG ; KO Validated
rat	WB	Cell Prolif; SCARF1 promotes M2 polarization of Kupffer cells via calcium-dependent PI3K-AKT-STAT3 signalling to improve liver transplantation.; Authors - Xue-Song Xu
human	FC	Front Immunol; Neutrophil heterogeneity in complement C1q expression associated with sepsis mortality; Authors - Alissa Trzeciak
mouse		Cell Rep; Fibroblast-Derived STC-1 Modulates Tumor-Associated Macrophages and Lung Adenocarcinoma Development.; Authors - Tamihiro Kamata